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Comparability of microbiota of swabbed and spit saliva
Author(s) -
Kaan Amke Marije,
Brandt Bernd W,
Buijs Mark J,
Crielaard Wim,
Keijser Bart JF,
Zaura Egija
Publication year - 2022
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12858
Subject(s) - saliva , comparability , medicine , biology , mathematics , combinatorics
In general, saliva is used for microbiota analysis in longitudinal studies, and several collection methods are being used. Using a robust sample collection procedure is important, as it may influence salivary composition. This study explored the comparability of the microbiota of swabbed and spit saliva. Twenty‐two females participated in this cross‐sectional study. The bacterial composition of the three saliva samples (swab collected by the participant (SW‐P), swab collected by the researcher (SW‐R), and spit (SP) was assessed by 16S rRNA gene amplicon sequencing. The bacterial composition of the swabbed and the spit saliva was significantly different irrespective of the operator, and Shannon diversity was significantly higher in spit saliva than in SW‐P and SW‐R. The salivary microbiota of spit and swabbed adult saliva differs significantly. Research on microbial composition therefore requires collection of similar saliva sample types in all study participants.