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The effect of phytic acid on enzymatic degradation of dentin
Author(s) -
Forgione Diletta,
Nassar Mohannad,
SeseogullariDirihan Roda,
Thitthaweerat Suppason,
TezvergilMutluay Arzu
Publication year - 2021
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12771
Subject(s) - phytic acid , phosphoric acid , dentin , chemistry , n terminal telopeptide , incubation , cathepsin , type i collagen , acid phosphatase , biochemistry , enzyme , dentistry , medicine , alkaline phosphatase , organic chemistry , osteocalcin
We evaluated the effect of phytic acid on matrix metalloproteinase (MMP)‐ or cysteine cathepsin (CC)‐mediated dentin degradation. Demineralized dentin beams were divided into five groups (n = 12) and treated with 1%, 2%, or 3% phytic acid or with 37% phosphoric acid. Untreated demineralized beams served as controls. After incubation for 1 or 3 wk, dry mass loss was determined and aliquots of incubation media were analysed for cross‐linked telopeptide of type I collagen (ICTP) fragments for MMP‐mediated and c‐terminal telopeptide of type I collagen (CTX) for cathepsin‐k‐mediated degradation. The direct effect of phytic acid was evaluated using MMP activity assay. Data were analysed using repeated‐measures anova . ICTP releases with 1% and 2% phytic acid treatment were statistically significantly lower than those following phosphoric acid treatment at 3 wk. The CTX release for phytic acid‐treated beams at 3 wk was not significantly different from that of untreated control beams, but it was significantly lower than that of phosphoric acid‐treated beams. Their MMP activities at 3 wk were not significantly different from those of the controls but they were significantly lower than those seen for phosphoric acid‐treated beams. Compared to phosphoric acid, phytic acid treatment resulted in a reduced dentinal host‐derived endogenous enzymatic activity and collagen degradation.