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The role of USP34 in the fixation of titanium implants in murine models
Author(s) -
Xue Hanxiao,
Guo Yuchen,
Zhang Shiwen,
Xu Tong,
Wen Junru,
Kang Ning,
Yuan Quan
Publication year - 2020
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12696
Subject(s) - maxilla , fixation (population genetics) , femur , implant , mesenchymal stem cell , osseointegration , chemistry , dentistry , pathology , medicine , surgery , biochemistry , gene
Ubiquitin‐specific protease 34 (USP34), a member of the ubiquitin‐specific protease family, regulates osteogenic differentiation of bone marrow mesenchymal stem cells via bone morphogenetic protein signaling. This study aimed to investigate the role of USP34 in fixation of titanium implants in mouse models. Eight‐week‐old Usp34 ‐knockout ( Prx1‐Cre;Usp34 f/f ) mice and their Usp34 wild‐type ( Usp34 f/f ) control littermates were used. Experimental titanium implants were inserted into the distal ends of femurs and the edentulous area of maxillae. Two and four weeks after surgery, samples of femur and maxilla were obtained, and micro‐computed tomography scanning, histomorphometric analyses, and push‐in tests were performed on the samples. Compared with controls, Prx1‐Cre;Usp34 f/f mice showed reduced bone volume for both femurs and maxillae; a decreased femoral bone–implant contact ratio (BIC) at 2 wk [mean (standard error of the mean): 62.17% (2.15%) vs. 44.06% (3.45%)] and 4 wk [72.46% (1.61%) vs. 64.53% (1.93%)]; decreases in femoral bone volume fraction (BV/TV) and push‐in resistance; and lower BIC and BV/TV of the maxillae. Taken together, our data demonstrate that specific deletion of Usp34 in mesenchymal stem cells impairs fixation of titanium implants in mice.