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In vivo biocompatibility and biomineralization of calcium silicate cements
Author(s) -
Benetti Francine,
GomesFilho João E.,
Araújo Lopes Juliana M.,
Barbosa Jéssica G.,
Jacinto Rogério C.,
Cintra Luciano T. A.
Publication year - 2018
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12539
Subject(s) - mineral trioxide aggregate , von kossa stain , biocompatibility , biomineralization , capsule , haematoxylin , eosin , chemistry , materials science , dentistry , biomedical engineering , pathology , staining , chemical engineering , medicine , biology , biochemistry , alkaline phosphatase , botany , engineering , enzyme , organic chemistry
A new mineral trioxide aggregate (MTA) material has been developed with a modified composition that requires investigations to support its clinical use. This study evaluated the biocompatibility and biomineralization of this new MTA material and compared it with that of two other MTA cements over time. Tubes containing materials (or empty tubes as controls) were inserted into the subcutaneous tissues of 40 rats. On days 7, 15, 30, 60, and 90, the tubes were removed with the surrounding tissues, which were either stained with haematoxylin and eosin or von Kossa for further analyses or unstained for observation under polarized light. On days 7 and 15, moderate inflammation was observed in most specimens, and the fibrous capsule was thick. On day 30, there was mild inflammation in all groups, and the fibrous capsule was thin. On days 60 and 90, there was mild inflammation in the material groups, while the control group showed no inflammation, although no statistically significant difference between the groups was observed and the fibrous capsule was thin. All material groups showed structures that stained with von Kossa and could be observed under polarized light; this was not found for the control. In conclusion, the new MTA material had biocompatibility and biomineralization properties similar to those of the two existing MTA materials.