Premium
Effect of non‐thermal atmospheric plasma on the dentin‐surface topography and composition and on the bond strength of a universal adhesive
Author(s) -
Ayres Ana P.,
Bonvent Jean J.,
Mogilevych Borys,
Soares Luis E. S.,
Martin Airton A.,
Ambrosano Glaucia M.,
Nascimento Fábio D.,
Van Meerbeek Bart,
Giannini Marcelo
Publication year - 2018
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12388
Subject(s) - dentin , bond strength , molar , chemistry , adhesive , dentistry , raman spectroscopy , nuclear chemistry , materials science , composite material , layer (electronics) , medicine , physics , optics , organic chemistry
This study investigated the effect of application of non‐thermal atmospheric plasma ( NTAP ) on the topography and composition of the dentin surface, as well as the microtensile bond strength ( μ TBS ) of a universal adhesive to NTAP ‐treated dentin. Exposed flat dentin surfaces from human third molars were either treated with NTAP for 10 and 30 s or untreated (control). The dentin‐surface topography and chemical composition were characterized by atomic force microscopy ( n = 3) and Raman confocal spectroscopy ( n = 5), respectively. The μ TBS ( n = 8) of Scotchbond Universal to dentin was determined after storage for 24 h and 1 yr, either by direct water exposure or under simulated pulpal pressure. In‐situ zymography was used to evaluate the influence of NTAP on the dentin‐enzymatic activity. Non‐thermal atmospheric plasma produced no remarkable topographical or chemical alterations at the dentin surface; only the amount of phosphate decreased following 10 s of treatment with NTAP . After 1 yr of direct water exposure, the μ TBS of NTAP ‐treated specimens did not differ statistically significantly from that of untreated controls, whereas simulated pulpal pressure‐aging resulted in a significantly higher μ TBS for NTAP ‐treated dentin. The dentin‐enzymatic activity appeared to be treatment‐dependent, but the untreated controls showed more intense fluorescence within the hybrid layer. Scotchbond Universal maintained its μ TBS strength after 1 yr of direct water exposure and simulated pulpal pressure, although remarkable statistical differences between treatments were observed depending on the aging condition.