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Interaction between calcitonin gene‐related peptide‐immunoreactive neurons and satellite cells via P2Y 12 R in the trigeminal ganglion is involved in neuropathic tongue pain in rats
Author(s) -
Sugawara Shiori,
Okada Shinji,
Katagiri Ayano,
Saito Hiroto,
Suzuki Tatsuro,
Komiya Hiroki,
Kanno Kohei,
Ohara Kinuyo,
Iinuma Toshimitsu,
Toyofuku Akira,
Iwata Koichi
Publication year - 2017
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12382
Subject(s) - calcitonin gene related peptide , lingual nerve , trigeminal ganglion , medicine , neuropathic pain , endocrinology , receptor , tongue , chemistry , neuroscience , anesthesia , biology , neuropeptide , pathology , sensory system
The P2Y 12 receptor expressed in satellite cells of the trigeminal ganglion is thought to contribute to neuropathic pain. The functional interaction between neurons and satellite cells via P2Y 12 receptors and phosphorylated extracellular signal‐regulated kinase 1/2 ( pERK 1/2) underlying neuropathic pain in the tongue was evaluated in this study. Expression of P2Y 12 receptor was enhanced in pERK 1/2‐immunoreactive cells encircling trigeminal ganglion neurons after lingual nerve crush. The administration to lingual nerve crush rats of a selective P2Y 12 receptor antagonist, MRS 2395, attenuated tongue hypersensitivity to mechanical and heat stimulation and suppressed the increase in the relative numbers of calcitonin gene‐related peptide ( CGRP )‐immunoreactive neurons and neurons encircled by pERK 1/2‐immunoreactive cells. Administration of the P2Y 1,12,13 receptor agonist, 2‐(methylthio)adenosine 5′‐diphosphate trisodium salt hydrate (2‐Me SADP ), to naïve rats induced neuropathic pain in the tongue, as in lingual nerve crush rats. Co‐administration of 2‐Me SADP + MRS 2395 to naïve rats did not result in hypersensitivity of the tongue. The relative number of CGRP ‐immunoreactive neurons increased following this co‐administration, but to a lesser degree than observed in 2‐MeSADP‐administrated naïve rats, and the relative number of neurons encircled by pERK 1/2‐immunoreactive cells did not change. These results suggest that the interaction between activated satellite cells and CGRP ‐immunoreactive neurons via P2Y 12 receptors contributes to neuropathic pain in the tongue associated with lingual nerve injury.