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Orthodontically induced osteocyte apoptosis under different force magnitudes in rats: an immunohistochemical study
Author(s) -
Kassem Hassan E.,
Talaat Iman M.,
ElSawa Afaf,
Ismail Hanan,
Zaher Abbas
Publication year - 2017
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12366
Subject(s) - osteocyte , molar , apoptosis , chemistry , immunohistochemistry , bite force quotient , maxillary molar , orthodontics , endocrinology , medicine , dentistry , anatomy , osteoblast , biochemistry , in vitro
We investigated the effect of different force magnitudes on osteocyte apoptosis in a model of orthodontic tooth movement. Forty‐nine male Sprague Dawley rats (7–9 wk of age) were divided into light‐ and heavy‐force groups ( n = 21 each group) and a control group ( n = 7). A coil spring delivered pressure (either 10–15 g or 20–25 g) to the left maxillary first molar. The rats were sacrificed 1, 3, or 5 d after placement of the appliance. Sections of the maxillary first molars were immunostained for caspase‐3. Upon force application, the number of apoptotic osteocytes significantly increased in the pressure side at 1 d and remained the same at 3 d and 5 d. However, there was no significant difference in the number of apoptotic osteocytes between the two force groups. We conclude that osteocyte apoptosis appears to increase under orthodontic loading, reaching a plateau after 1 d. However, osteocyte apoptosis seems to be independent of the magnitude of orthodontic forces tested.