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Effect of a one‐step self‐etch adhesive on endogenous dentin matrix metalloproteinases
Author(s) -
Apolonio Fabianni M.,
Mazzoni Annalisa,
Angeloni Valeria,
Scaffa Polliana M. C.,
Santi Spartaco,
Saboia Vicente de Paulo A.,
Tay Franklin R.,
Pashley David H.,
Breschi Lorenzo
Publication year - 2017
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12337
Subject(s) - dentin , zymography , adhesive , matrix metalloproteinase , chemistry , smear layer , dentinal tubule , matrix (chemical analysis) , materials science , dentistry , layer (electronics) , composite material , biochemistry , medicine
Degradation of the hybrid layer created in dentin by dentin adhesives is caused by enzyme activities present within the dentin matrix that destroy unprotected collagen fibrils. The aim of the present study was to evaluate the effect of a one‐step self‐etch adhesive system on dentinal matrix metalloproteinases 2 and 4 ( MMP ‐2 and MMP ‐9, respectively) using in situ zymography and an enzymatic activity assay. The null hypothesis tested was that there are no differences in the activities of dentinal MMP s before and after treatment with a one‐step adhesive system. The MMP ‐2 and MMP ‐9 activities in dentin treated with the one‐step adhesive, Adper Easy Bond, were quantified using an enzymatic activity assay system. The MMP activities within the hybrid layer created by the one‐step adhesive tested were also evaluated using in situ zymography. The enzymatic assay revealed an increase in MMP ‐2 and MMP ‐9 activities after treatment with adhesive. In situ zymography indicated that gelatinolytic activity is present within the hybrid layer created with the one‐step self‐etch adhesive. The host‐derived gelatinases were localized within the hybrid layer and remained active after the bonding procedure. It is concluded that the one‐step self‐etch adhesive investigated activates endogenous MMP ‐2 and MMP ‐9 with the dentin matrix, which may cause collagen degradation over time.