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Intrafibrillar mineralization of polyacrylic acid‐bound collagen fibrils using a two‐dimensional collagen model and Portland cement‐based resins
Author(s) -
Wu Shiyu,
Gu Lisha,
Huang Zihua,
Sun Qiurong,
Chen Huimin,
Ling Junqi,
Mai Sui
Publication year - 2017
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12319
Subject(s) - polyacrylic acid , simulated body fluid , portland cement , apatite , dentin , mineralization (soil science) , chemistry , desorption , adsorption , materials science , chemical engineering , cement , composite material , nuclear chemistry , mineralogy , polymer , organic chemistry , nitrogen , engineering
The biomimetic remineralization of apatite‐depleted dentin is a potential method for enhancing the durability of resin–dentin bonding. To advance this strategy from its initial proof‐of‐concept design, we sought to investigate the characteristics of polyacrylic acid ( PAA ) adsorption to desorption from type I collagen and to test the mineralization ability of PAA ‐bound collagen. Portland cement and β ‐tricalcium phosphate ( β ‐ TCP ) were homogenized with a hydrophilic resin blend to produce experimental resins. The collagen fibrils reconstituted on nickel (Ni) grids were mineralized using different methods: (i) group I consisted of collagen treated with Portland cement‐based resin in simulated body fluid ( SBF ); (ii) group II consisted of PAA ‐bound collagen treated with Portland cement‐based resin in SBF ; and (iii) group III consisted of PAA ‐bound collagen treated with β ‐ TCP ‐doped Portland cement‐based resin in deionized water. Intrafibrillar mineralization was evaluated using transmission electron microscopy. We found that a carbonyl‐associated peak at pH 3.0 increased as adsorption time increased, whereas a hydrogen bond‐associated peak increased as desorption time increased. The experimental resins maintained an alkaline pH and the continuous release of calcium ions. Apatite was detected within PAA ‐bound collagen in groups II and III . Our results suggest that PAA ‐bound type I collagen fibrils can be mineralized using Portland cement‐based resins.