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Sensitive detection of Porphyromonas gingivalis based on magnetic capture and upconversion fluorescent identification with multifunctional nanospheres
Author(s) -
Qin Wei,
Zheng Bin,
Yuan Yuan,
Li Meng,
Bai Yang,
Chang Jin,
Wang Hanjie,
Wang Yonglan
Publication year - 2016
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12286
Subject(s) - porphyromonas gingivalis , detection limit , fluorescence , chemistry , monoclonal antibody , microbiology and biotechnology , bacteria , materials science , biology , antibody , chromatography , physics , optics , immunology , genetics
A specific and sensitive detection system was designed to detect Porphyromonas gingivalis , a major periodontal pathogen, in mixed bacterial fluids. This new detection system was based on the use of fluorescent and magnetic encoding nanospheres that were conjugated with monoclonal antibodies specific to P. gingivalis , thus enabling rapid detection of the target bacterium. This strategy simplifies the detection process and improves the sensitivity compared with conventional methods, with a detection limit of approximately 10 colony‐forming units (CFU) ml −1 . This new method shows strong anti‐interference ability and excellent selectivity and specificity to detect P. gingivalis in mixed solutions.