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Localization of cystic fibrosis transmembrane conductance regulator signaling complexes in human salivary gland striated duct cells
Author(s) -
Zinn Vina Z.,
Khatri Aditi,
Mednieks Maija I.,
Hand Arthur R.
Publication year - 2015
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12184
Subject(s) - cystic fibrosis transmembrane conductance regulator , ezrin , microbiology and biotechnology , apical membrane , immunogold labelling , chemistry , protein subunit , submandibular gland , protein kinase a , transmembrane protein , biology , kinase , cell , biochemistry , endocrinology , membrane , anatomy , cytoskeleton , receptor , ultrastructure , gene
The cystic fibrosis transmembrane conductance regulator ( CFTR ) is a cyclic AMP ‐dependent protein kinase ( PKA )‐regulated Cl − channel, crucial for epithelial cell regulation of salt and water transport. Previous studies showed that ezrin, an actin binding and A‐kinase anchoring protein ( AKAP ), facilitates association of PKA with CFTR . We used immunohistochemistry and immunogold transmission electron microscopy to localize CFTR , ezrin, and PKA type II regulatory ( RII ) and catalytic (C) subunits in striated duct cells of human parotid and submandibular glands. Immunohistochemistry localized the four proteins mainly to the apical membrane and the apical cytoplasm of striated duct cells. In acinar cells, ezrin localized to the luminal membrane, and PKA RII subunits were present in secretory granules, as previously described. Immunogold labeling showed that CFTR and PKA RII and C subunits were localized to the luminal membrane and associated with apical granules and vesicles of striated duct cells. Ezrin was present along the luminal membrane, on microvilli and along the junctional complexes between cells. Double labeling showed specific protein associations with apical granules and vesicles and along the luminal membrane. Ezrin, CFTR , and PKA RII and C subunits are co‐localized in striated duct cells, suggesting the presence of signaling complexes that serve to regulate CFTR activity.