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Effect of different desensitizers on inhibition of bovine dentin demineralization: micro‐computed tomography assessment
Author(s) -
Lodha Ena,
Hamba Hidenori,
Nakashima Syozi,
Sadr Alireza,
Nikaido Toru,
Tagami Junji
Publication year - 2014
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12155
Subject(s) - demineralization , dentistry , saliva , chemistry , remineralisation , dentin , dentin hypersensitivity , medicine , enamel paint , biochemistry
This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro‐computed tomography ( μ CT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish (Duraphat, a positive control), a calcium phosphate desensitizer (Teethmate Desensitizer), and a fluoro‐alumino‐calcium silicate‐based desensitizer (Nanoseal). After each treatment, the specimens in Duraphat, Nanoseal, and Teethmate Desensitizer groups were pre‐immersed in artificial saliva ( pH 6.5) for either 1 d or 1 wk. The mineral loss of the specimens after demineralization ( pH 5.0, 3 h) was evaluated by μ CT. The treated surface was investigated with scanning electron microscopy. Mineral loss in all treatment groups was significantly lower than that in DW. Duraphat was the most effective treatment against demineralization, followed by Nanoseal. Nanoseal showed significantly better reduction in mineral loss following immersion for 1 wk in artificial saliva than for 1 d. However, Teethmate Desensitizer and Duraphat did not exhibit enhanced inhibition of demineralization over a longer period of immersion in artificial saliva. Scanning electron microscopy images showed deposition of particles on the dentin in both Teethmate Desensitizer. The application of Teethmate Desensitizer and Nanoseal to the exposed dentin surface resulted in inhibition of demineralization, with Nanoseal resulting in improved inhibition after prolonged immersion in artificial saliva.