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Matrix metalloproteinase inhibitory properties of benzalkonium chloride stabilizes adhesive interfaces
Author(s) -
Sabatini Camila,
Patel Shaival K.
Publication year - 2013
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12089
Subject(s) - benzalkonium chloride , adhesive , dentin , chemistry , chlorhexidine , dentistry , chromatography , medicine , organic chemistry , layer (electronics)
This study evaluated the effects of different concentrations of benzalkonium chloride ( BAC ) on the preservation of adhesive interfaces created with two etch‐and‐rinse adhesives and its inhibitory properties on dentin matrix metalloproteinase ( MMP ) activity. The following groups were tested with the adhesive systems Optibond Solo Plus and All‐Bond 3: Group 1, adhesive without inhibitor (control); Group 2, topical 2.0% chlorhexidine (2.0% CHX ); Group 3, phosphoric acid with 1.0%wt BAC ( BAC ‐ PA ); Group 4, 0.25% BAC ‐adhesive (0.25% BAC ); Group 5, 0.5% BAC ‐adhesive (0.5% BAC ); Group 6, 1.0% BAC ‐adhesive (1.0% BAC ); and Group 7, 2.0% BAC ‐adhesive (2.0% BAC ). Composite cylinders were fabricated, and shear bond strength ( SBS ) was evaluated after 24 h, 6 months, and 18 months of storage. Extracts from concentrated demineralized human dentin powder were subjected to SDS ‐ PAGE and incubated in the presence of 0.25, 0.5, 1.0, and 2.0% BAC . Overall, stable bonds were maintained for 18 months. Improved bond strengths were seen for 0.5% BAC and 1.0% BAC when bonding with Optibond Solo Plus, and for 0.25% BAC and 0.5% BAC when bonding with All‐Bond 3. Zymographic analysis revealed complete inhibition of gelatinolytic activity with BAC . Benzalkonium chloride, at all concentrations, inhibited dentin proteolytic activity, which seems to have contributed to the improved bond stability after 18 months for specific combinations of BAC concentration and adhesive.