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Expression of Clu and Tgfb1 during murine tooth development: effects of in‐vivo transfection with anti‐mi R ‐214
Author(s) -
Khan QalbESaleem,
Sehic Amer,
Khuu Cuong,
Risnes Steinar,
Osmundsen Harald
Publication year - 2013
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12056
Subject(s) - clusterin , ameloblast , biology , microbiology and biotechnology , amelogenesis , transfection , epithelium , odontoblast , gene , enamel paint , pathology , apoptosis , genetics , pulp (tooth) , medicine , dentistry
Expression of clusterin ( C lu ) in the murine first molar tooth germ was markedly increased at postnatal developmental stages. The time‐course of expression of this gene paralleled those of other genes encoding proteins involved during the secretory phase of odontogenesis, as described previously. Immunohistochemical studies of clusterin in murine molar tooth germs suggested this protein to be located in outer enamel epithelium, regressing enamel organ, secretory ameloblasts, and the dental epithelium connecting the tooth to the oral epithelium at an early eruptive stage. Immunolabelling of transforming growth factor beta‐1 ( TGF ‐β1) revealed it to be located close to clusterin. The levels of expression of C lu and T gfb1 were markedly decreased following in‐vivo transfection with anti‐mi R ‐214. In contrast, the expression of several genes associated with regulation of growth and development were increased by this treatment. We suggest that clusterin has functions during secretory odontogenesis and the early eruptive phase. Bioinformatic analysis after treatment with anti‐mi R ‐214 suggested that, whilst cellular activities associated with tooth mineralization and eruption were inhibited, activities associated with an alternative developmental activity (i.e. biosynthesis of contractile proteins) appeared to be stimulated. These changes probably occur through regulation mediated by a common cluster of transcription factors and support suggestions that micro RNA s (mi RNA s) are highly significant as regulators of differentiation during odontogenesis.