Premium
Effect of metalloporphyrins on red autofluorescence from oral bacteria
Author(s) -
Volgenant Catherine M. C.,
Veen Monique H.,
Soet Johannes J.,
Cate Jacob M.
Publication year - 2013
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12045
Subject(s) - actinomyces naeslundii , autofluorescence , fusobacterium nucleatum , microbiology and biotechnology , chemistry , dental plaque , streptococcus mutans , enterococcus faecalis , anaerobic bacteria , fusobacterium , bacteria , biochemistry , biology , porphyromonas gingivalis , bacteroides , fluorescence , genetics , physics , quantum mechanics , escherichia coli , gene
The aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. Bacteria were grown anaerobically on tryptic soy agar ( TSA ) supplemented with nutrients, including magnesium‐porphyrins from spinach and iron‐porphyrins from heme. The autofluorescence was then assessed at 405 nm excitation. On the TSA without additives, no autofluorescence was observed from any of the species tested. On the TSA containing sheep blood, red autofluorescence was observed only from P arvimonas micra . When the TSA was supplemented with blood, hemin, and vitamin K , red autofluorescence was observed from A ctinomyces naeslundii, B ifidobacterium dentium , and S treptococcus mutans . Finally, on the TSA supplemented with spinach extract, red autofluorescence was observed from A ggregatibacter actinomycetemcomitans , A . naeslundii , E nterococcus faecalis , F usobacterium nucleatum, L actobacillus salivarius, S . mutans , and V eillonella parvula . We conclude that the bacteria related to dental caries and periodontal disease exhibit red autofluorescence. The autofluorescence characteristics of the tested strains depended on the nutrients present, such as metalloporphyrins, suggesting that the metabolic products of the oral biofilm could be responsible for red autofluorescence.