Premium
CIITA rs4774 and rs6498122 polymorphisms are associated with oral lichen planus in C hinese people: a case–control study
Author(s) -
Wu Dan,
Wang Lifeng,
Sun Mao,
Wang Guoxia,
Fu Shanmin,
Dong Guangying,
Wu Yuanming
Publication year - 2013
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/eos.12029
Subject(s) - ciita , snp , single nucleotide polymorphism , genotype , allele , genetics , pathogenesis , immunology , major histocompatibility complex , biology , medicine , gene , mhc class ii
Oral lichen planus ( OLP ) is a common autoimmune inflammatory disorder that is difficult to cure, and its pathogenesis is still largely unknown. The major histocompatibility complex ( MHC ) class II transactivator ( CIITA ) gene has been reported to be an important candidate in some classical autoimmune diseases, and certain single nucleotide polymorphisms ( SNP s) in CIITA have been confirmed to be associated with susceptibility to some autoimmune diseases. We conducted this research to investigate the existence of any correlation between OLP and SNP s in CIITA . A case–control study was performed to genotype 15 SNP s in the CIITA gene from 42 patients with OLP and from 86 controls; this was carried out by the PCR and then by a locus‐specific single‐base extension reaction. Allele detection was performed using matrix‐assisted laser desorption/ionization time‐of‐flight ( MALDI ‐ TOF ) mass spectrometry. The SNP rs4774 variant in exon 11 (+1614 G / C , G ly500 A la) of CIITA is significantly associated with OLP in healthy persons, both in genotype frequency and in allele frequency. Another intronic SNP , rs6498122, showed significant differences only in allele frequency. In conclusion, our data show that the two SNP s rs4774 and rs6498122 are associated with OLP and could also indicate the autoimmune characteristics of OLP .