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Construction of a bacterial artificial chromosome library of Endoclita excrescens as a tool for comparative gene mapping in Lepidoptera
Author(s) -
Okumura Azusa,
Kobayashi Masanori,
Kitajima Hiroshi,
Yasukochi Yuji,
Suzuki Go,
Sahara Ken
Publication year - 2019
Publication title -
entomological science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 28
eISSN - 1479-8298
pISSN - 1343-8786
DOI - 10.1111/ens.12350
Subject(s) - biology , bacterial artificial chromosome , lepidoptera genitalia , synteny , bombyx mori , chromosome , fluorescence in situ hybridization , genetics , gene , karyotype , genomic library , genome , botany , base sequence
Karyotype evolution in one of the most diverse and species‐rich group of insects, moths and butterflies (Lepidoptera), has interesting features that remain to be resolved. Recent studies showed that fluorescence in situ hybridization using bacterial artificial chromosome clones (BAC‐FISH) is an efficient cytogenetic method for identification and gene mapping of lepidopteran chromosomes. Using comparative mapping by BAC‐FISH, extensive synteny of genes was revealed between chromosomes of different lepidopteran species based on Bombyx mori genomic information. However, this comparative mapping has been done only in representatives of advanced groups of Lepidoptera. Here we constructed a BAC library of Endoclita excrescens , which belongs to the primitive lepidopteran family Hepialidae. High molecular weight DNA for the library construction was prepared from the pupae by using a rapid nuclear isolation method known in plants. The BAC clones of E. excrescens contain 66.6 kb inserts on average. The successful application of BAC‐FISH showed that the BAC library of E. excrescens is a useful tool for comparative gene mapping on chromosomes of this species.