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First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae)
Author(s) -
Bugrov Alexander G.,
Ilinsky Yury Yu.,
Strunov Anton,
Zhukova Mariya,
Kiseleva Elena,
Akimoto Shinichi,
Tatsuta Haruki
Publication year - 2016
Publication title -
entomological science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 28
eISSN - 1479-8298
pISSN - 1343-8786
DOI - 10.1111/ens.12187
Subject(s) - biology , acrididae , wolbachia , grasshopper , orthoptera , locus (genetics) , karyotype , zoology , genetics , x chromosome , chromosome , evolutionary biology , ecology , gene
The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographic analysis of the chromosome races and subraces confirms the genetic divergence of the races and subraces in P. sapporensis . Here we first report that P. sapporensis is infected with Wolbachia consisting of three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation of cell tissue of P. sapporensis using electron microscopy confirmed the infection of Wolbachia that was inferred from polymerase chain reaction and revealed the distribution of the bacteria in the head, thorax and abdomen of P. sapporensis embryos. Our finding may shed new light on Wolbachia as a possible agent causing hybrid dysfunction resulting from experimental crosses between chromosome races or subraces of P. sapporensis .