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Isolation and characterization of microsatellite loci from three cryptic species of Ceratosolen emarginatus
Author(s) -
Yang LiYuan,
Yu Hui,
Zhang DaYong,
Liao WanJin
Publication year - 2016
Publication title -
entomological science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 28
eISSN - 1479-8298
pISSN - 1343-8786
DOI - 10.1111/ens.12179
Subject(s) - biology , species complex , microsatellite , linkage disequilibrium , inbreeding , reproductive isolation , loss of heterozygosity , evolutionary biology , population , genetics , allele , haplotype , gene , phylogenetic tree , demography , sociology
From an enriched (AG) n /(AC) n library, we developed nine polymorphic microsatellite loci for three cryptic species of the fig‐pollinating wasp Ceratosolen emarginatu s. We genotyped one population for each of the three cryptic species across all the nine loci. In total, 204 alleles were detected from the three cryptic species of C . emarginatus . The observed heterozygosity was 0.755 ± 0.034, 0.653 ± 0.030 and 0.603 ± 0.073 in C . emarginatus populations A, B and C, respectively; the expected heterozygosity was 0.850 ± 0.031, 0.724 ± 0.035 and 0.702 ± 0.104, respectively. No linkage disequilibrium was found between any two loci of all three cryptic species. The newly isolated microsatellite markers will be very useful for estimating the genetic variation within and among the cryptic species and for revealing the mechanisms of speciation and inbreeding coexistence hypothesis of the cryptic species.