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Thirteen microsatellite loci for L aodelphax striatellus and cross amplification in related taxa
Author(s) -
Liu Yudi,
Zhang Baoqin,
Hou Maolin
Publication year - 2014
Publication title -
entomological science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 28
eISSN - 1479-8298
pISSN - 1343-8786
DOI - 10.1111/ens.12044
Subject(s) - biology , pyrosequencing , microsatellite , genetics , allele , expressed sequence tag , population , gene , genome , demography , sociology
With the advent of next‐generation R oche 454 pyrosequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene‐based molecular markers. In a previous study more than 1450 simple sequence repeats ( SSR s) in expressed sequence tag ( EST ) sequences resulting from 454 pyrosequencing of L aodelphax striatellus c DNA were identified. From these we developed PCR primers for 40 di‐ or tri‐candidate SSR s (minimum repeats > 8) from the combined EST library. After extensive optimization, 13 pairs were end labeled with a fluorescent dye. Here, we also tested these 13 SSR s for cross‐species amplification in two other planthoppers, N ilaparvata lugens and S ogatella furcifera . The marker transferability was considerably high in N . lugens (92.31%) and in S . furcifera (61.54%). All of the 13 fluorescent SSR s were polymorphic, with allele numbers ranging from two to seven for 24 male and 24 female L . striatellus individuals. Observed heterozygosities ranged from 0.016 to 0.621. Development of SSR markers from EST s will be a valuable tool to improve our understanding of population structure in this important rice pest.