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Head‐to‐head comparison of amplified plasmonic exosome Aβ42 platform and single‐molecule array immunoassay in a memory clinic cohort
Author(s) -
Tanaka Tomotaka,
Ruifen Joyce Chong,
Nai YingHwey,
Tan Chin Hong,
Lim Carine Z. J.,
Zhang Yan,
Stephenson Mary C.,
Hilal Saima,
Saridin Francis N.,
Gyanwali Bibek,
Villaraza Steven,
Robins Edward G.,
Ihara Masafumi,
Schöll Michael,
Zetterberg Henrik,
Blennow Kaj,
Ashton Nicholas J.,
Shao Huilin,
Reilhac Anthonin,
Chen Christopher
Publication year - 2021
Publication title -
european journal of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 124
eISSN - 1468-1331
pISSN - 1351-5101
DOI - 10.1111/ene.14704
Subject(s) - medicine , cohort , biomarker , positron emission tomography , oncology , clinical trial , cohort study , dried blood spot , pathology , nuclear medicine , biochemistry , chemistry , biology , genetics
Background and purpose Various blood biomarkers reflecting brain amyloid‐β (Aβ) load have recently been proposed with promising results. However, to date, no comparative study amongst blood biomarkers has been reported. Our objective was to examine the diagnostic performance and cost effectiveness of three blood biomarkers on the same cohort. Methods Using the same cohort ( n = 68), the performances of the single‐molecule array (Simoa) Aβ40, Aβ42, Aβ42/Aβ40 and the amplified plasmonic exosome (APEX) Aβ42 blood biomarkers were compared using amyloid positron emission tomography (PET) as the reference standard. The extent to which these blood tests can reduce the recruitment cost of clinical trials was also determined by identifying amyloid positive (Aβ+) participants. Results Compared to Simoa biomarkers, APEX‐Aβ42 showed significantly higher correlations with amyloid PET retention values and excellent diagnostic performance (sensitivity 100%, specificity 93.3%, area under the curve 0.995). When utilized for clinical trial recruitment, our simulation showed that pre‐screening with blood biomarkers followed by a confirmatory amyloid PET imaging would roughly half the cost (56.8% reduction for APEX‐Aβ42 and 48.6% for Simoa‐Aβ42/Aβ40) compared to the situation where only PET imaging is used. Moreover, with 100% sensitivity, APEX‐Aβ42 pre‐screening does not increase the required number of initial participants. Conclusions With its high diagnostic performance, APEX is an ideal candidate for Aβ+ subject identification, monitoring and primary care screening, and could efficiently enrich clinical trials with Aβ+ participants whilst halving recruitment costs.