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Change from paddy rice to vegetable growing changes nitrogen‐cycling microbial communities and their variation with depth in the soil
Author(s) -
Qin H. L.,
Zhang Z. X.,
Lu J.,
Zhu Y. J.,
Webster R.,
Liu X. L.,
Yuan H. Z.,
Hou H. J.,
Chen C. L.,
Wei W. X.
Publication year - 2016
Publication title -
european journal of soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.244
H-Index - 111
eISSN - 1365-2389
pISSN - 1351-0754
DOI - 10.1111/ejss.12365
Subject(s) - topsoil , cycling , nitrogen cycle , paddy field , agronomy , nitrification , terminal restriction fragment length polymorphism , abundance (ecology) , nitrate , microbial population biology , soil ph , soil microbiology , mineralization (soil science) , nutrient cycle , ecosystem , environmental science , nitrogen , soil water , chemistry , biology , ecology , restriction fragment length polymorphism , bacteria , polymerase chain reaction , history , genetics , archaeology , organic chemistry , gene , biochemistry
Changes in land use are likely to affect the abundance and functioning of microorganisms in the soil. In C hina many paddy fields are being converted for vegetable growing. We wished to determine how these changes affected the microbial populations in one particular region in H unan province, where the climate is subtropical monsoon, as an example. We sampled the soil down to 1 m in several fields: three that were still growing paddy rice, three that had been converted for vegetable growing 2 years earlier and three that had been growing vegetables for 25 years. Quantitative polymerase chain reaction ( qPCR ) and terminal‐restriction fragment length polymorphism ( T‐RFLP ) were used to determine the abundance and community composition of nxrA ‐containing nitrifiers and narG ‐containing denitrifiers in the soil. The abundances of these organisms depended largely on the amount of organic matter in the soil and decreased with increasing depth, as did the potential nitrification rate ( PNR ) and nitrate reductase activity ( NRA ). Enzyme activity was significantly correlated with the abundance of nitrogen‐cycling bacteria. The change from rice to vegetable growing resulted in more residual nitrate‐ N in the soil, which correlated more strongly with the abundance of nxrA ‐containing nitrifiers in the topsoil (0–20 cm) and narG ‐containing denitrifiers in the deeper soil (80–100 cm). In general, the numbers of nitrogen‐cycling microorganisms decreased markedly with increasing depth, but were less affected by the change from rice to vegetable cultivation in the fields investigated. Our results suggest that the abundances of nitrogen‐cycling microbial communities are affected more by depth in the soil than by change of land use in these circumstances. Highlights The abundance and function of soil microorganisms change when paddy fields are converted to vegetable growing. Residual nitrate‐ N in the soil is affected by nitrifiers in topsoil and denitrifiers in the deeper soil. Their abundances depend largely on the amount of organic matter in soil and decrease with increasing depth. The genetic structures of the microorganisms depend more on depth in the soil than on change from rice to vegetable growing.