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Acute physical exercise increases APPL 1/ PI 3K signaling in the hypothalamus of lean mice
Author(s) -
Gaspar Rafael Calais,
Muñoz Vitor Rosetto,
Kuga Gabriel Keine,
Nakandakari Susana Castelo Branco Ramos,
Crisol Barbara Moreira,
Lenhare Luciene,
Breda Leonardo,
Botezelli José Diego,
Sant'Ana Marcella Ramos,
Silva Adelino S. R.,
Cintra Dennys Esper,
Moura Leandro Pereira,
Ropelle Eduardo Rochete,
Pauli José Rodrigo
Publication year - 2019
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.14490
Subject(s) - hypothalamus , adiponectin , endocrinology , medicine , pleckstrin homology domain , energy homeostasis , signal transduction , adipokine , signal transducing adaptor protein , pi , chemistry , biology , insulin , microbiology and biotechnology , receptor , obesity , leptin , biochemistry , insulin resistance
Adiponectin is an adipokine that acts in the control of energy homeostasis. The adaptor protein containing the pleckstrin homology domain, phosphotyrosine‐binding domain, and leucine zipper motif 1 ( APPL 1) is a key protein in the adiponectin signaling. The APPL 1 mediates a positive effect on the insulin signaling through the interaction with the phosphoinositide 3‐kinase ( PI 3K). Thus, the present study aimed to explore the effects of an acute physical exercise session on the hypothalamic adiponectin signaling. Firstly, using bioinformatics analysis, we found a negative correlation between hypothalamic APPL 1 mRNA levels and food consumption in several strains of genetically diverse BXD mice. Also, the mice and the human database revealed a positive correlation between the levels of APPL 1 mRNA and PI 3K mRNA . At the molecular level, the exercised mice showed increased APPL 1 and PI 3K (p110) protein contents in the hypothalamus of Swiss mice. Furthermore, the exercise increases co‐localization between APPL 1 and PI 3K p110 predominantly in neurons of the arcuate nucleus of hypothalamus ( ARC ). Finally, we found an acute exercise session reduced the food intake 5 hr after the end of fasting. In conclusion, our results indicate that physical exercise reduces the food intake and increases some proteins related to adiponectin pathway in the hypothalamus of lean mice.