Premium
Rostro‐caudal maturation of glial cells in the accessory olfactory system during development: involvement in outgrowth of Gn RH neurites
Author(s) -
Geller Sarah,
Lomet Didier,
Caraty Alain,
Tillet Yves,
Duittoz Anne,
Vaudin Pascal
Publication year - 2017
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.13732
Subject(s) - neurite , microbiology and biotechnology , biology , neurogenesis , olfactory ensheathing glia , forebrain , rostral migratory stream , glial fibrillary acidic protein , astrocyte , olfactory bulb , progenitor cell , neuroscience , central nervous system , subventricular zone , immunology , stem cell , immunohistochemistry , in vitro , biochemistry
During mammalian embryonic development, Gn RH neurones differentiate from the nasal placode and migrate through the nasal septum towards the forebrain. We previously showed that a category of glial cells, the olfactory ensheathing cells ( OEC ), forms the microenvironment of migrating Gn RH neurones. Here, to characterize the quantitative and qualitative importance of this glial, we investigated the spatiotemporal maturation of glial cells in situ and the role of maturing glia in Gn RH neurones development ex vivo . More than 90% of migrating Gn RH neurones were found to be associated with glial cells. There was no change in the cellular microenvironment of Gn RH neurones in the regions crossed during embryonic development as glial cells formed the main microenvironment of these neurones (53.4%). However, the phenotype of OEC associated with Gn RH neurones changed across regions. The OEC progenitors immunoreactive to brain lipid binding protein formed the microenvironment of migrating Gn RH neurones from the vomeronasal organ to the telencephalon and were also present in the diencephalon. However, during Gn RH neurone migration, maturation of OEC to [ GFAP +] state (glial fibrillary acid protein) was only observed in the nasal septum. Inducing depletion of OEC in maturation, using transgenic mice expressing herpes simplex virus thymidine kinase driven by the GFAP promoter, had no impact on neurogenesis or on triggering Gn RH neurones migration in nasal explant culture. Nevertheless, depletion of [ GFAP +] cells decreased Gn RH neurites outgrowth by 57.4%. This study suggests that specific maturation of OEC in the nasal septum plays a role in morphological differentiation of Gn RH neurones.