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Issue Cover (October 2015)
Publication year - 2015
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12988
Subject(s) - dopaminergic , dopamine transporter , green fluorescent protein , dopamine , genetically modified mouse , neuroscience , biology , transgene , midbrain , microbiology and biotechnology , gene , central nervous system , genetics
Cover Illustration: eGFP expression in a novel dopamine transporter transgenic mouse line ( Dat1‐eGFP ) is selective for dopaminergic neurons. Immunohistochemical quantification of eGFP reporter distribution in ventral midbrain revealed that a vast majority islocalized to dopaminergic neurons. Behavioral and functional characterization further showed that hemizygous Dat1‐eGFP miceare not phenotypically aberrant when compared to wildtype controls. Postnatal dopaminergic neurons from Dat1‐eGFP mice were sorted using fluorescence‐activated cell sorting and could be cultured for up to two weeks, suggesting that this mouse strain constitutes a promising tool for delineating new aspects of dopamine biology. Eur. J. Neurosci ., 42 , 2438–2454.