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Layer‐specific endocannabinoid‐mediated long‐term depression of GABA ergic neurotransmission onto principal neurons in mouse visual cortex
Author(s) -
Sun Wenjuan,
Wang Laijian,
Li Shuo,
Tie Xiaoxiu,
Jiang Bin
Publication year - 2015
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12958
Subject(s) - neurotransmission , neuroscience , inhibitory postsynaptic potential , endocannabinoid system , postsynaptic potential , gabaa receptor , glutamatergic , chemistry , biology , receptor , glutamate receptor , biochemistry
Visually induced endocannabinoid‐mediated long‐term depression of GABA ergic neurotransmission ( iLTD ) mediates the maturation of GABA ergic release in layer 2/3 of visual cortex. Here we examined whether the maturation of GABA ergic transmission in other layers of visual cortex also requires endocannabinoids. The developmental plasticity of GABA ergic neurotransmission onto the principal neurons in different layers of mouse visual cortex was examined in cortical slices by whole‐cell recordings of inhibitory postsynaptic currents evoked by presynaptic inhibitory inputs. Theta burst stimulation of GABA ergic inputs induced an endocannabinoid‐mediated long‐term depression of GABA ergic neurotransmission onto pyramidal cells in layer 2/3 from postnatal day (P)10 to 30 and in layer 5 from P10 to 40, whereas that of GABA ergic inputs did not induce iLTD onto star pyramidal neurons in layer 4 at any time postnatally, indicating that this plasticity is laminar‐specific. The developmental loss of iLTD paralleled the maturation of GABA ergic inhibition in both layer 2/3 and layer 5. Visual deprivation delayed the developmental loss of iLTD in layers 3 and 5 during a critical period, while 2 days of light exposure eliminated iLTD in both layers. Furthermore, the GABA ergic synapses in layers 2/3 and 5 did not normally mature in the type 1 cannabinoid receptor knock‐out mice, whereas those in layer 4 did not require endocannabinoid receptor for maturation. These results suggest that visually induced endocannabinoid‐dependent iLTD mediates the maturation of GABA ergic release in extragranular layer rather than in granular layer of mouse visual cortex.