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The Drosophila Kctd‐family homologue Kctd12‐like modulates male aggression and mating behaviour
Author(s) -
Williams Michael J.,
Goergen Philip,
Phad Ganesh,
Fredriksson Robert,
Schiöth Helgi B.
Publication year - 2014
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12619
Subject(s) - biology , g protein coupled receptor , drosophila (subgenus) , receptor , aggression , 5 ht receptor , mating , neuroscience , serotonin , genetics , psychology , developmental psychology , gene
In Drosophila, serotonin (5‐ HT ) regulates aggression, mating behaviour and sleep/wake behaviour through different receptors. Currently, how these various receptors are themselves regulated is still not completely understood. The KCTD 12‐family of proteins, which have been shown to modify G‐protein‐coupled receptor ( GPCR ) signalling in mammals, are one possibility of auxiliary proteins modulating 5‐ HT receptor signalling. The KCTD 12‐family was found to be remarkably conserved and present in species from C. elegans to humans. The Drosophila KCTD 12 homologue Kctd12‐like ( Ktl ) was highly expressed in both the larval and adult CNS . By performing behavioural assays in male Drosophila , we now reveal that Ktl is required for proper male aggression and mating behaviour. Previously, it was shown that Ktl is in a complex with the Drosophila 5‐ HT receptor 5‐ HT 7, and we observed that both Ktl and the 5‐ HT 1A receptor are required in insulin‐producing cells ( IPC s) for proper adult male behaviour, as well as for hyperaggressive activity induced by the mammalian 5‐ HT 1A receptor agonist 8‐hydroxy‐2‐dipropylaminotetralin‐hydrobromide. Finally, we show that Ktl expression in the IPC s is necessary to regulate locomotion and normal sleep/wake patterns in Drosophila , but not the 5‐ HT 1A receptor. Similar to what was observed with mammalian KCTD 12‐family members that interact physically with a GPCR receptor to regulate desensitization, in Drosophila Ktl may function in GPCR 5‐ HT receptor pathways to regulate their signalling, which is required for proper adult male behaviour.