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Peroxisome proliferator‐activated receptor gamma‐coactivator‐1 alpha coordinates sphingolipid metabolism, lipid raft composition and myelin protein synthesis
Author(s) -
Camacho Alberto,
Huang Jeffrey K.,
DelintRamirez Ilse,
Yew Tan Chong,
Fuller Maria,
Lelliott Christopher J.,
VidalPuig Antonio,
Franklin Robin J. M.
Publication year - 2013
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12281
Subject(s) - sphingolipid , myelin , coactivator , lipid metabolism , peroxisome proliferator activated receptor alpha , biology , ganglioside , lipid raft , biochemistry , peroxisome proliferator activated receptor , receptor , microbiology and biotechnology , chemistry , nuclear receptor , endocrinology , central nervous system , transcription factor , gene
Peroxisome proliferator‐activated receptor gamma‐coactivator‐1 alpha ( PGC 1a) is involved in energy and lipid metabolism, and its loss leads to neurodegenerative changes in the striatum. Here we performed lipidomic analysis on brain extracts from PGC 1a mutant and wild‐type mice. We found increased phosphatidylcholine and decreased ceramides in the brain of PGC 1a‐deficient mice. An analysis of lipid raft fractions revealed increased ceramide, glucocylceramides and GM 1 ganglioside in the PGC 1a mutants. In the cerebellum, we observed a decrease in proteins associated with myelination, but were unable to detect any morphological abnormalities in compact myelin formation in PGC 1a mutants compared with wild‐type mice. Although PGC 1a is involved in lipid biosynthesis, we concluded that altered lipid composition in the PGC 1a mutant did not directly affect central nervous system myelin morphology.