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Antibodies to cannabinoid type 1 receptor co‐react with stomatin‐like protein 2 in mouse brain mitochondria
Author(s) -
Morozov Yury M.,
Dominguez Martin H.,
Varela Luis,
Shanabrough Marya,
Koch Marco,
Horvath Tamas L.,
Rakic Pasko
Publication year - 2013
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12237
Subject(s) - mitochondrion , cannabinoid , polyclonal antibodies , cannabinoid receptor , antibody , receptor , endocannabinoid system , microbiology and biotechnology , chemistry , biology , biochemistry , immunology , antagonist
Abstract Anti‐cannabinoid type 1 receptor ( CB 1 ) polyclonal antibodies are widely used to detect the presence of CB 1 in a variety of brain cells and their organelles, including neuronal mitochondria. Surprisingly, we found that anti‐ CB 1 sera, in parallel with CB 1 , also recognize the mitochondrial protein stomatin‐like protein 2. In addition, we show that the previously reported effect of synthetic cannabinoid WIN 55,212‐2 on mitochondrial complex III respiration is not detectable in purified mitochondrial preparations. Thus, our study indicates that a direct relationship between endocannabinoid signaling and mitochondrial functions in the cerebral cortex seems unlikely, and that caution should be taken interpreting findings obtained using anti‐ CB 1 antibodies.