Premium
Upregulation of the neuronal P er‐ A rnt‐ S im domain protein 4 ( N pas4) in the rat corticolimbic system following focal cerebral ischemia
Author(s) -
Leong Wai Khay,
Klaric Thomas S.,
Lin Yingxi,
Lewis Martin D.,
Koblar Simon A.
Publication year - 2013
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12163
Subject(s) - downregulation and upregulation , ischemia , neuroscience , neuroplasticity , middle cerebral artery , immunohistochemistry , brain ischemia , chemistry , in vivo , psychology , microbiology and biotechnology , biology , medicine , biochemistry , gene
The neuronal P er‐ A rnt‐ S im domain protein 4 ( N pas4) is an important transcriptional regulator of synaptic plasticity and cognition. The present study characterises the in vivo neuroanatomical expression pattern of the N pas4 protein in a rat model of focal cerebral ischemia. Animals were subjected to unilateral middle cerebral artery occlusion for 2 h, after which the spatiotemporal and neuronal profiles of N pas4 protein expression were analysed by immunohistochemistry at different time points post‐reperfusion. Focal cerebral ischemia induced an early, transient and robust upregulation of N pas4 in a brain region‐dependent manner involving predominantly principal neurons. Interestingly, we observed a unique differential induction of N pas4 protein expression in corticolimbic regions of the rat brain that are critically linked to cognition and emotion. These findings suggest that stroke‐induced N pas4 upregulation may be involved in a transcriptional regulatory program within the corticolimbic circuitry following an ischemic insult.