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Reelin and the Cdc42/Rac1 guanine nucleotide exchange factor α PIX /Arhgef6 promote dendritic Golgi translocation in hippocampal neurons
Author(s) -
Meseke Maurice,
Rosenberger Georg,
Förster Eckart
Publication year - 2013
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12153
Subject(s) - reelin , golgi apparatus , dab1 , reeler , microbiology and biotechnology , dendritic spine , guanine nucleotide exchange factor , chromosomal translocation , biology , hippocampal formation , chemistry , neuroscience , gtpase , endoplasmic reticulum , biochemistry , gene , extracellular matrix
In the cerebral cortex of reeler mutant mice lacking reelin expression, neurons are malpositioned and display misoriented apical dendrites. Neuronal migration defects in reeler have been studied in great detail, but how misorientation of apical dendrites is related to reelin deficiency is poorly understood. In wild‐type mice, the Golgi apparatus transiently translocates into the developing apical dendrite of radially migrating neurons. This dendritic Golgi translocation has recently been shown to be promoted by reelin. However, the underlying signalling mechanisms are largely unknown. Here, we show that the Cdc42/Rac1 guanine nucleotide exchange factor α PIX /Arhgef6 promoted translocation of Golgi cisternae into developing dendrites of hippocampal neurons. Reelin treatment further increased the α PIX ‐dependent effect. In turn, overexpression of exchange activity‐deficient α PIX or dominant‐negative (dn) Cdc42 or dn‐Rac1 impaired dendritic Golgi positioning, an effect that was not compensated by reelin treatment. Together, these data suggest that α PIX may promote dendritic Golgi translocation, as a downstream component of a reelin‐modulated signalling pathway. Finally, we found that reelin promoted the translocation of the Golgi apparatus into the dendrite that was most proximal to the reelin source. The distribution of reelin may thus contribute to the selection of the process that becomes the apical dendrite.

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