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mi RNA ‐15a, mi RNA ‐16, mi RNA ‐126, mi RNA ‐146a, and mi RNA ‐223 expressions in autologous hematopoietic stem cell transplantation and their impact on engraftment
Author(s) -
Nowicki Mateusz,
Szemraj Janusz,
Wierzbowska Agnieszka,
Misiewicz Małgorzata,
Małachowski Roman,
Pluta Agnieszka,
Grzybowska–Izydorczyk Olga,
Robak Tadeusz,
SzmigielskaKapłon Anna
Publication year - 2018
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.13036
Subject(s) - microrna , haematopoiesis , transplantation , stem cell , homing (biology) , hematopoietic stem cell transplantation , rna , hematopoietic stem cell , angiogenesis , immunology , cancer research , biology , medicine , microbiology and biotechnology , gene , ecology , biochemistry
Objective Micro RNA s engaged in angiogenesis and hematopoiesis can influence hematopoietic stem cells ( HSC s) homing after transplantation by targeting bone marrow niche microenvironment. This study aimed to examine the kinetics of mi RNA ‐15a, mi RNA ‐16, mi RNA ‐126, mi RNA ‐146a, and mi RNA ‐223 in autologous HSC transplantation settings. Methods The study comprised of 51 patients with hematological malignancies (42 multiple myeloma, 9 lymphoma). Samples were taken at four time points: before conditioning, after chemotherapy but prior to autologous HSC transplantation (day 0), on day +7, and +14 days after HSCT . The mi RNA levels were evaluated by the real‐time PCR method. Results A significant, steady decline of all tested micro RNA s in the course of transplantation, as compared to the baseline, was found. The study revealed that higher levels of mi RNA ‐15a, mi RNA ‐16, mi RNA ‐126, and mi RNA ‐146a on day 0 correlated with longer time to engraftment. Additionally, a positive correlation between the levels of mi RNA ‐15a, mi RNA ‐146a, and mi RNA ‐223 assessed on day +7 and the time to engraftment was observed. Conclusions In conclusion, all investigated micro RNA s changed significantly in the course of transplantation. Our results suggest that the mi RNA s may participate in hematopoietic recovery in the early post‐transplant period and influence engraftment efficiency after HSCT .