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Expression pattern of PD ‐L1 and PD ‐L2 in classical Hodgkin lymphoma, primary mediastinal large B‐cell lymphoma, and gray zone lymphoma
Author(s) -
Tanaka Yosuke,
Maeshima Akiko Miyagi,
Nomoto Junko,
Makita Shinichi,
Fukuhara Suguru,
Munakata Wataru,
Maruyama Dai,
Tobinai Kensei,
Kobayashi Yukio
Publication year - 2018
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.13033
Subject(s) - lymphoma , pathology , polysomy , immunohistochemistry , antibody , staining , reed–sternberg cell , fluorescence in situ hybridization , biology , in situ hybridization , medicine , immunology , gene expression , gene , biochemistry , hodgkin lymphoma , chromosome
Objectives We aimed at investigating the relationship between classical Hodgkin lymphoma ( cHL ), primary mediastinal large B‐cell lymphoma ( PMBL ), and gray zone lymphoma ( GZL ) with intermediate characteristics between cHL and PMBL , from the perspective of the aberration in programed cell death 1 and the programed death ligands ( PDL s) network. Methods We explored the expression levels of PDL s and chromosomal anomalies in 67 cases: 34 cases with cHL , 20 with PMBL , and 13 with GZL , using immunohistochemical analyses and Fluorescence In Situ Hybridization ( FISH ). Results Twenty‐one cHL (62%), 3 PMBL (15%), and 6 GZL (46%) cases showed staining to PD ‐L1 antibodies in more than 70% of tumor cells. Two cHL (6%), 10 PMBL (50%), and 3 GZL (23%) cases were not stained by PD ‐L1 antibodies. Patients over 40 years old manifest more frequent expression of PD ‐L1 in cHL . Proportion of tumors stained by PD ‐L2 antibody was increased in PMBL . FISH analyses with a PD ‐L1/ PD ‐L2 probe detected 5 amplification, 1 gain, and 7 polysomy cases in cHL , 1 amplification and 1 polysomy case in GZL , and amplification in 1 PMBL case. Conclusion We identified increased staining of PD ‐L1 in cHL and that of PD ‐L2 in PMBL . GZL had a pattern similar to that of cHL .