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Detection of inherited chromosomally integrated HHV ‐6 (ci HHV ‐6) in a marker chromosome
Author(s) -
Campioni Diana,
Gentili Valentina,
Cavazzini Francesco,
Bortolotti Daria,
Nacheva Elisabeth P.,
Cuneo Antonio,
Di Luca Dario,
Rizzo Roberta
Publication year - 2017
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12872
Subject(s) - biology , chromosome , peripheral blood mononuclear cell , gene , microbiology and biotechnology , immunology , genetics , in vitro
Objectives Inherited chromosomally integrated human herpesvirus‐6 (ci HHV ‐6) is characterised by the complete HHV ‐6 genome integration into the host germ line genome and is vertically transmitted with a Mendelian inheritance. By now, the only relationship between ci HHV ‐6 and diseases seems to be with angina pectoris. Methods We report a case of an 82‐year‐old man diagnosed with diffuse large B‐cell lymphoma ( DLBCL ) on October 2014. To substantiate the suspicion of ci HHV ‐6, we analysed peripheral blood mononuclear cells, bone marrow biopsy and pleural effusion‐derived mesothelial cells with PCR , RT ‐ PCR and FISH . Results Virological routine screening by PCR showed the absence of HHV ‐8 and EBV infections, while the presence of HHV ‐6 DNA (ie, U22, U42 and U94 HHV ‐6 genes), with a viral load of about 1.0 genome per cell, strongly suggests ci HHV ‐6. The RT ‐ PCR showed the positivity only for the immediate‐early U94, at low levels of transcription (100±15 transcripts/1 μg RNA ). FISH analysis reported a case of inherited ci HHV ‐6 in 17p chromosome region and, for the first time, in a marker chromosome. Conclusions This is the first case of inherited ci HHV ‐6 in a marker chromosome, possibly elucidating the role of this abnormality in the biology of DLBCL .