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Similar chemokine receptor profiles in lymphomas with central nervous system involvement – possible biomarkers for patient selection for central nervous system prophylaxis, a retrospective study
Author(s) -
Lemma Siria A.,
Pasanen Anna Kaisa,
Haapasaari KirsiMaria,
Sippola Antti,
Sormunen Raija,
Soini Ylermi,
Jantunen Esa,
Koivunen Petri,
Salokorpi Niina,
Bloigu Risto,
TurpeenniemiHujanen Taina,
Kuittinen Outi
Publication year - 2016
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12626
Subject(s) - cxcr5 , cxcl13 , lymphoma , pathology , c c chemokine receptor type 7 , lymph node , immunoelectron microscopy , chemokine receptor , primary central nervous system lymphoma , cxcr4 , medicine , biology , immunohistochemistry , chemokine , immunology , inflammation
Central nervous system ( CNS ) relapse occurs in around 5% of diffuse large B‐cell lymphoma ( DLBCL ) cases. No biomarkers to identify high‐risk patients have been discovered. We evaluated the expression of lymphocyte‐guiding chemokine receptors in systemic and CNS lymphomas. Immunohistochemical staining for CXCR 4, CXCR 5, CCR 7, CXCL 12, and CXCL 13 was performed on 89 tissue samples, including cases of primary central nervous system lymphoma ( PCNSL ), secondary CNS lymphoma ( sCNSL ), and systemic DLBCL . Also, 10 reactive lymph node samples were included. Immunoelectron microscopy was performed on two PCNSL s, one sCNSL , one systemic DLBCL , and one reactive lymph node samples, and staining was performed for CXCR 4, CXCR 5, CXCL 12, and CXCL 13. Chi‐square test was used to determine correlations between clinical parameters, diagnostic groups, and chemokine receptor expression. Strong nuclear CXCR 4 positivity correlated with systemic DLBCL , whereas strong cytoplasmic CXCR 5 positivity correlated with CNS involvement ( P = 0.003 and P = 0.039). Immunoelectron microscopy revealed a nuclear CXCR 4 staining in reactive lymph node, compared with cytoplasmic and membranous localization seen in CNS lymphomas. We found that CNS lymphoma presented a chemokine receptor profile different from systemic disease. Our findings give new information on the CNS tropism of DLBCL and, if confirmed, may contribute to more effective targeting of CNS prophylaxis among patients with DLBCL.