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Analysis of multipotent mesenchymal stromal cells used for acute graft‐versus‐host disease prophylaxis
Author(s) -
Kuzmina Larisa A.,
Petinati Nataliya A.,
Shipounova Iri.,
Sats Natalia V.,
Bigildeev Alexey E.,
Zezina Ekaterina A.,
Popova Maria D.,
Drize Nina J.,
Parovichnikova Ele.,
Savchenko Valery G.
Publication year - 2016
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12613
Subject(s) - mesenchymal stem cell , medicine , haematopoiesis , colony forming unit , bone marrow , transplantation , pdgfrb , stromal cell , cell , immunology , andrology , cancer research , biology , pathology , stem cell , gene , biochemistry , genetics , bacteria
Background Multipotent mesenchymal stromal cells ( MSC s) are used for prophylaxis of acute graft‐versus‐host disease ( aGvHD ) after allogeneic hematopoietic cell transplantation (allo‐ HCT ). Not all samples of MSC are efficient for aGvHD prevention. The suitability of MSC s for aGvHD prophylaxis was studied. Methods MSC s were derived from the bone marrow ( BM ) of HCT donor and cultivated for no more than three passages. The characteristics of donor BM samples including colony‐forming unit fibroblast ( CFU ‐F) concentration, growth parameters of MSC s, and the relative expression levels ( REL ) of different genes were analyzed. MSC s were injected intravenously precisely at the moment of blood cell reconstitution. Results MSC s infusion induced a significant threefold decrease in aGvHD development and improved overall survival compared with the standard prophylaxis group. In ineffective MSC samples (9.4%), a significant decrease in total cell production and the REL of CSF 1, FGFR 1, and PDGFRB was observed. In all studied BM samples, the cumulative MSC production and CFU ‐F concentrations decreased with age. The expression levels of FGFR 2, PPARG , and VEGF differed by age. Conclusions A universal single indicator for the prediction of MSC eligibility for aGvHD prophylaxis was not identified. A multiparameter mathematical model for selecting MSC samples effective for the prevention of aGvHD was proposed.