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Genomic analysis of clonal eosinophils by CGH arrays reveals new genetic regions involved in chronic eosinophilia
Author(s) -
Arefi Maryam,
Robledo Cristina,
Peñarrubia María J.,
Coca Alfonso García,
Cordero Miguel,
HernándezRivas Jesús M.,
García Juan Luis
Publication year - 2014
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12379
Subject(s) - pdgfra , myeloproliferative neoplasm , eosinophilia , myeloid , comparative genomic hybridization , biology , immunology , neurofibromatosis , medicine , pathology , cancer research , genetics , chromosome , bone marrow , gist , myelofibrosis , gene , stromal cell
To assess the presence of genetic imbalances in patients with myeloproliferative neoplasms (MPNs), 38 patients with chronic eosinophilia were studied by array comparative genomic hybridization ( aCGH ): seven had chronic myelogenous leukaemia (CML), BCR‐ABL1 positive, nine patients had myeloproliferative neoplasia Ph− (MPN‐Ph−), three had a myeloid neoplasm associated with a PDGFRA rearrangement, and the remaining two cases were Lymphoproliferative T neoplasms associated with eosinophilia. In addition, 17 patients had a secondary eosinophilia and were used as controls. Eosinophilic enrichment was carried out in all cases. Genomic imbalances were found in 76% of all MPN patients. Losses on 20q were the most frequent genetic abnormality in MPNs (32%), affected the three types of MPN studied. This study also found losses at 11q13.3 in 26% of patients with MPN‐Ph− and in 19p13.11 in two of the three patients with an MPN associated with a PDGFRA rearrangement. In addition, 29% of patients with CML had losses on 8q24. In summary, aCGH revealed clonality in eosinophils in most MPNs, suggesting that it could be a useful technique for defining clonality in these diseases. The presence of genetic losses in new regions could provide new insights into the knowledge of these MPN associated with eosinophilia.