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Immature platelet fraction measured on the S ysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation
Author(s) -
Linden Noreen,
Klinkenberg Lieke J.J.,
Meex Steven J.R.,
Beckers Erik A.M.,
Wit Norbert C.J.,
Prinzen Lenneke
Publication year - 2014
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12319
Subject(s) - hemocytometer , stem cell , transplantation , platelet , andrology , immunology , medicine , chemistry , biology , microbiology and biotechnology , pathology
Objectives A period of thrombocytopenia is common after stem cell transplantation ( SCT ). To prevent serious bleeding complications, prophylactic platelet transfusions are administered. Previous studies have shown that a rise in immature platelets precedes recovery of platelet count. Our aim was to define a cutoff value for immature platelets predicting thrombopoietic recovery within 2 d. Methods Hematological parameters were measured on the S ysmex XN hemocytometer. We calculated reference change values ( RCV ) for platelets in eight healthy individuals as marker for platelet recovery. To define a cutoff value, we performed ROC analysis using data from 16 autologous SCT patients. Results RCV for platelet concentration was 14.1%. Platelet recovery was observed 13 (median; range 9–31) days after SCT . Increase in immature platelet fraction (IPF) before platelet recovery was seen in all autologous SCT patients. Optimal cutoff IPF was found to be 5.3% for platelet recovery within 2 d (specificity 0.98, sensitivity 0.47, positive predictive value 0.93). Conclusions We identified an optimal cutoff value for IPF 5.3% to predict platelet recovery after autologous SCT within 2 d. Implementing this cutoff value in transfusion strategy may reduce the number of prophylactic platelet transfusions.