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Acquisition of t(11;14) in a patient with chronic lymphocytic leukemia carrying both t(14;19)(q32;q13.1) and +12
Author(s) -
Nishida Yuhki,
Takeuchi Kengo,
Tsuda Kenji,
Ugai Tomotaka,
Sugihara Hiroki,
Yamakura Masayuki,
Takeuchi Masami,
Matsue Kosei
Publication year - 2013
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12119
Subject(s) - chronic lymphocytic leukemia , medicine , leukemia
A rare recurrent chromosomal translocation, t(14;19)(q32;q13), has been identified in a variety of B‐cell malignancies, including chronic lymphocytic leukemia ( CLL ). We report a unique case of CLL in a patient carrying both trisomy 12 and t(14;19) (q32;q13.1), in whom t(11;14)(q13;q32) developed at relapse. The patient was a 77‐yr‐old woman, and her lymphoma cells at presentation showed CD 5 + , CD 10 − , CD 19 + , CD 20 + (dim), CD 23 + , CD 38 + , and CD 11c + . At relapse, the patient's lymphoma cells showed positive staining for cyclin D1 in addition to CD 5, CD 20, and CD 23. Lymphoma cells in specimens at both presentation and relapse were positive for lymphoid enhancer factor 1 ( LEF 1) and negative for sex‐determining region Y‐box 11 ( SOX 11). IGH ‐ BCL 1 FISH became positive at relapse. Split FISH assay using BCL 1 , BCL 3 , IGH , and CCND 1 probes on lymph node specimens obtained at presentation and at autopsy confirmed that the translocation of BCL 3 was solely detected in the lymph node at presentation and detected BCL 3 and CCND 1 translocations in the specimen at autopsy. These observations indicated that IGH ‐ BCL 3 and IGH ‐ CCND 1 had occurred in the same clone after treatment of the disease. In line with immunohistochemical and cytogenetic studies, additional PCR analysis of the FR 3‐ JH region showed the same sequence derived from IGHV 4‐34 in specimens obtained at disease onset and relapse.