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Some viral micro RNA s were up‐regulated in megakaryocytes incubated with immune thrombocytopenia plasma
Author(s) -
Wang Ya,
Huang Rui,
Song Chaoyang,
Hu Haiyan,
Zhang Meixia
Publication year - 2013
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/ejh.12063
Subject(s) - megakaryocytopoiesis , megakaryocyte , thrombopoietin , flow cytometry , rna , haematopoiesis , biology , virus , pathogenesis , platelet , peripheral blood mononuclear cell , immune system , microarray , viral load , immunology , microarray analysis techniques , real time polymerase chain reaction , gene expression , virology , gene , stem cell , microbiology and biotechnology , in vitro , genetics
Objectives Micro RNA s (mi RNA s) have been shown to play important roles in hematopoiesis, such as megakaryocytopoiesis. Although immature megakaryocytes in bone marrow are one of the most typical characteristics of immune thrombocytopenia ( ITP ) which is often preceded by viral infection, there has been so far no report on the relationship between viral mi RNA s, maturity of megakaryocytes, and ITP . This study aims to investigate whether viral mi RNA s are involved in the pathogenesis of ITP , for example, by modulating the production of megakaryocytes. Methods Plasma samples from adult patients with acute ITP or healthy volunteers were added to the culture system wherein umbilical cord blood mononuclear cells were proliferating into megakaryocytes. Flow cytometry and electron microscope were used to identify the differences between the two groups of megakaryocytes. Microarray analysis and real‐time quantitative PCR (q RT‐PCR ) were used to quantify the expression levels of viral mi RNA s. Bioinformatics analysis of the microarray results was performed. Results The yield and maturity of megakaryocytes were significantly repressed in the presence of ITP plasma compared with the control: (11.89 ± 1.62)% vs. (33.61 ± 3.24)% ( P < 0.001). The expression levels of 14 viral mi RNA s were significantly up‐regulated in the ITP group, five of which were further verified using qRT ‐ PCR ( P < 0.05). Bioinformatics analysis to the 14 up‐regulated viral mi RNA s indicated that most of the predicted target genes of viral mi RNA s were related to cell development, metabolic process, and biosynthesis, which might be related to mechanism of maturation defect of megakaryocytes. Conclusion Expression levels of certain viral mi RNA s can be up‐regulated by the treatment with plasma from ITP patients, indicating viral mRNA s may be involved in the pathogenesis of ITP, possibly by regulating megakaryocytopoiesis.