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First report of 16Sr II‐C subgroup phytoplasma association with Acacia mangium in Tripura, India
Author(s) -
Rao Govind Pratap,
Bahadur Amar,
Das Sukhen Chandra,
Ranebennur Hemavati,
Mitra Surabhi,
Kumar Manish,
Kumar Shailender
Publication year - 2020
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12573
Subject(s) - acacia mangium , phytoplasma , biology , 16s ribosomal rna , phylogenetic tree , restriction fragment length polymorphism , amplicon , genetic analysis , sequence analysis , botany , veterinary medicine , horticulture , gene , polymerase chain reaction , genetics , medicine
Leaf yellowing symptoms were observed on Acacia mangium in the Sipahijala district of Tripura, India, during June 2017. Symptomatic and asymptomatic leaf samples (three of each) were collected from roadside trees of A . mangium for DNA extraction using the CTAB method. Amplicons of ~1.25 kb and ~480 bp were detected in all the symptomatic samples using the phytoplasma‐specific universal 16S rRNA and sec A gene primers. Pair wise sequence analysis of 16S rRNA gene sequences, virtual RFLP and phylogenetic analysis revealed that the phytoplasma strain associated with A . mangium belonged to phytoplasma subgroup 16SrII‐C. This is the first report of an association between the 16SrII‐C subgroup and A . mangium leaf yellowing.