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A rapid and holistic approach to screen susceptibility of Prunus species to Armillaria root rot
Author(s) -
Devkota Pratima,
Hammerschmidt Raymond
Publication year - 2019
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12547
Subject(s) - armillaria , prunus , biology , rosaceae , botany , host (biology) , armillaria mellea , colonization , fungus , inoculation , horticulture , microbiology and biotechnology , ecology
We describe a holistic in vitro technique for inoculating roots of Prunus species with Armillaria solidipes, which is faster and more successful than previous methods. This method allows associated active and passive host defences to be assessed. Sterile root segments of three Prunus spp. were placed next to or on top of 14‐day‐old cultures of A. solidipes . At 21 day, the success of fungal penetration and length of the fungal colonization, and host responses were evaluated. Also, an anti‐ Armillaria and anti‐ Cladosporium activity detection assays were conducted by utilizing the root periderm, the first tissue that needs to be penetrated by fungus. These methods revealed the variation in the relative tolerance of three Prunus spp. to A. solidipes . The overall success of fungal colonization in the wounded and intact root, host defence, and antifungal activity significantly differed among three Prunus spp. Results indicate that this in vitro method can be used as a preliminary step in screening tree species to Armillaria spp.