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First report of Phytophthora pseudosyringae recovered from aquatic ecosystems in Bulgaria
Author(s) -
Christova Petya Koeva,
Lyubenova Aneta B.,
Kostov Kaloyan V.,
Slavov Slavtcho B.
Publication year - 2019
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12505
Subject(s) - biology , internal transcribed spacer , botany , aquatic ecosystem , pathogenicity , phytophthora , ecosystem , isolation (microbiology) , phytophthora ramorum , vaccinium , ecology , microbiology and biotechnology , ribosomal rna , biochemistry , gene
Two Phytophthora pseudosyringae isolates were recovered from aquatic ecosystems in Bulgaria during a two‐year investigation of Phytophthora distribution in water sources in the country. Isolate RVit2016/6d was derived from Boyana Lake at the Vitosha Mountain, whereas isolate RTr2016/32d was obtained from Erma River at the Ruy Mountain. Both isolates belong to P. pseudosyringae species according to their morphological and physiological characteristics, as well as to the DNA sequence analysis of the internal transcribed spacer (ITS) region. The pathogenicity of the isolates to wild cranberry plants ( Vaccinium vitis‐idea ) was studied by detached leaves experiments and in planta . Both P. pseudosyringae isolates were able to cause leaf necrosis and death of plants within 3 months. The ability of the pathogens to infect cranberry leaves at different temperatures was also investigated. The significance of P. pseudosyringae species and its potential threat for forest ecosystems is discussed. To our knowledge, this is the first report of P. pseudosyringae isolation in Bulgaria.