Premium
q PCR quantification of Ophiognomonia clavigignenti‐juglandacearum from infected butternut trees under different release treatments
Author(s) -
Tanguay P.,
Blais M.,
Potvin A.,
Stewart D.,
Walker D.,
NadeauThibodeau N.,
DesRochers P.,
Rioux D.
Publication year - 2018
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12418
Subject(s) - conidium , biology , taqman , canker , spore , pathogen , polymerase chain reaction , real time polymerase chain reaction , botany , horticulture , veterinary medicine , microbiology and biotechnology , genetics , gene , medicine
Summary The use of a molecular assay for quantifying conidia of Ophiognomonia clavigignenti‐juglandacearum , the fungal pathogen responsible of butternut canker, was investigated. Purified DNA from conidia collected on glass fibre filters of a passive rain collectors was quantified using a TaqMan real‐time quantitative polymerase chain reaction (q PCR ) assay. The q PCR assay could specifically discriminate the target species from all other North American known species of Ophiognomonia , and it was sensitive enough to repeatedly detect one conidium. A linear relationship between numbers of conidia and q PCR C t values was determined, and used to assess the sporulation of the pathogen under trees that were released to promote their vigour. In total, 977 samples of field‐captured conidia from 49 trees, at two locations, and from two successive growing seasons were analysed. No significant difference of sporulation was observed under control and release treatments. However, our results demonstrated that q PCR assay was reliable for detecting and quantifying O. clavigignenti‐juglandacearum from environmental samples, which will be useful to assess further control methods for this disease.