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Development of microsatellite markers for Thekopsora areolata, the causal agent of cherry spruce rust
Author(s) -
Capador H.,
Samils B.,
Olson Å.
Publication year - 2018
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12413
Subject(s) - biology , rust (programming language) , microsatellite , population , botany , genetic marker , horticulture , genetics , gene , allele , demography , sociology , computer science , programming language
Summary Cherry spruce rust is a fungal disease of Norway spruce cones caused by Thekopsora areolata and responsible for significant losses in seed production in Sweden and Finland. Here, we report the first set of nine microsatellites, which will allow an effective genetic fingerprinting of T. areolata . The markers were isolated using the FIASCO method and were characterized using DNA from 49 single aecia sampled from spruce cones in three different seed orchards in Sweden. Eight of the nine markers were shown to be polymorphic among the aecia. The markers were unlinked and are therefore suitable for future population genetic studies.