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Characterization of endophytic bacteria with plant growth promotion and biological control potential isolated from walnut trees
Author(s) -
Ghorbani S.,
Harighi B.
Publication year - 2018
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12403
Subject(s) - biology , pseudomonas , 16s ribosomal rna , siderophore , bacteria , pseudomonas syringae , botany , microbiology and biotechnology , endophyte , genetics
Summary In this study, a total of 68 endophytic bacteria were isolated from different tissues of walnut trees. About 55% and 22% of the strains had the ability to produce indole acetic acid and gibberellic acid, respectively. The capability of isolates to solubilize phosphate, growth on N‐free medium, siderophore, protease and lipase production was varied. Based on phenotypic grouping and plant growth promotion properties, twelve isolates were selected and 16S rRNA gene‐based phylogenetic analysis revealed that strains showed 99%–100% similarity to Pseudomonas , Bacillus , Arthrobacter , Roseomonas and Streptomyces genera. Amongst the selected strains, PS 12, KS 54, JS 66 and KS 74 showed root and shoot growth enhancement of poplar cutting. NS 70, KS 54 and PL 36 strains showed antagonistic activity against Pseudomonas syringae pv. syringae; RR 47, KS 74 and NR 69 strains had inhibition effects against Brenneria nigrifluens; and JS 66 and RR 26 strains had antagonistic activity against both phytopathogens under in vitro conditions. This is the first reported study to elucidate the endophytic bacterial diversity associated with walnut trees with beneficial attributes.