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Application of MALDI ‐ TOF mass spectrometry for in vitro identification of wood decay polypores
Author(s) -
Pristaš P.,
Kvasnová S.,
Gáperová S.,
Gašparcová T.,
Gáper J.
Publication year - 2017
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12352
Subject(s) - basidiomycota , polyporaceae , fomes , biology , mass spectrometry , botany , mycelium , ganoderma , chromatography , ganoderma lucidum , chemistry , food science
Summary Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF MS ) was used for the discrimination among wood decay fungal species of the genera Fomitopsis , Rhodofomes , Fomes and Ganoderma (Polyporaceae, Basidiomycota). For all taxa studied, distance levels as high as 400 were observed and intraspecies variability and reproducibility accounted for distance levels up to 150, indicating the applicability of this method for the identification of filamentous basidiomycete fungi. The method was found to work on in vitro mycelia.