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A multiplex PCR ‐based technique for identification of Biscogniauxia mediterranea and Obolarina persica causing charcoal disease of oak trees in Zagros forests
Author(s) -
Safaee D.,
Khodaparast S. A.,
Mirabolfathy M.,
Mousanejad S.
Publication year - 2017
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12330
Subject(s) - biology , botany , charcoal , bark (sound) , identification (biology) , ecology , materials science , metallurgy
Summary Zagros oak ( Quercus spp.) forests ( ZOF ) cover approximately 4 million hectares of the Zagros Mountains in Iran. Oak charcoal disease caused by Biscogniauxia mediterranea and Obolarina persica has recently increased in some regions of ZOF . Detection of these fungi in host tissue and identification of the anamorphs by traditional methods have limitations and difficulties which were overcome using two primers, OP 1 and OP 2, based on rDNA sequences of O. persica and used along with the specific primers MED 1 and MED 2 for B. mediterranea to develop a multiplex PCR . This method was used to correctly identify 1 pg of fungal DNA per 1 mg of inner bark tissues of Quercus brantii , Q. infectoria and Q. libani .