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Bursaphelenchus tusciae in Tunisia associated with Hylurgus ligniperda
Author(s) -
Mejri M.,
Fonseca L.,
Cardoso J. M. S.,
Ben Jamâa M. L.,
Abrantes I.
Publication year - 2016
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12297
Subject(s) - biology , internal transcribed spacer , ribosomal dna , curculionidae , phylogenetic tree , restriction fragment length polymorphism , botany , bark beetle , zoology , genetics , polymerase chain reaction , gene
Summary Bursaphelenchus tusciae is reported for the first time in Tunisia and North Africa, associated with the insect Hylurgus ligniperda Fabricius (Coleoptera: Curculionidae: Scolytinae). Nematode identification was based on restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA. Phylogenetic analysis revealed that Tunisian B. tusciae clusters together with two other B. tusciae isolates forming a separate group close to B. hildegardae and B. eggersi . As H. ligniperda is among maritime pine scolytids pests in Tunisia and is widely distributed in North Africa, this study is an important contribution to the knowledge of Bursaphelenchus species associated with bark beetles of pine forests in Tunisia and North Africa.