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Defence‐related gene expression of Hevea brasiliensis clones in response to the white rot pathogen, Rigidoporus microporus
Author(s) -
Oghenekaro A. O.,
Omorusi V. I.,
Asiegbu F. O.
Publication year - 2016
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12260
Subject(s) - biology , microbiology and biotechnology , pathogenesis related protein , phenylalanine ammonia lyase , wrky protein domain , jasmonic acid , downregulation and upregulation , myb , gene expression , pathogen , gene , transcriptome , peroxidase , genetics , biochemistry , enzyme
Summary Gene expression levels of pathogenesis‐related proteins ( PR 1, PR 3, PR 5, PR 8 and PR 9), cell wall modification genes ( PAL and expansin), signal transduction genes ( ACC oxidase, AOC , MAPK ) and a Myb transcription factor were compared in two Hevea brasiliensis budded clones ( RRIM 612 and PR 107) 5 weeks after wounding and artificial inoculation with the white rot fungus, Rigidoporus microporus . PR 3 class I chitinase was upregulated in RRIM 612 in response to the pathogen when compared with the wounded control. PR 9 class IV peroxidase was highly upregulated in PR 107. PR 1 and PR 8 were expressed more in wounded than in inoculated clones. Genes involved in ethylene and jasmonic acid signalling pathways and phenylalanine ammonia‐lyase ( PAL ) were upregulated almost equally in both clones. The Myb transcription factor was upregulated in RRIM 612, while MAPK was upregulated in PR 107 when compared to the wounded control. The predicted expansin‐like protein was upregulated 40‐fold in RRIM 612 when compared to the wounded control. The results demonstrate the variability in defence responses in different clones and provide the first set of defence genes expression profiles in the host–pathogen interaction of the white rot disease of rubber trees.